Gateway technology with clonase ii user manual

 · Gateway Cloning Technology - Instruction Manual 1. GATEWAY™ Cloning Technology Note: This product is covered by Limited Label Licenses (see Section ). By use of this product, you accept the terms and conditions of the Limited Label Licenses. 2. Gateway™ Technology is schematically represented below. The attB × attP reaction is mediated by Gateway™ BP Clonase™ II enzyme mix; the attL × attR reaction is mediated by Gateway™ LR Clonase™ II enzyme mix. ccdB is the F plasmid-encoded gene that inhibits growth of E. coli and “gene” represents any DNAFile Size: KB.  · GATEWAY Cloning Technology Instruction Manual, but does not provide rights to synthesize prime (pages, Mb) Gateway Computer Hardware DX Operation user’s manual (94 pages, Mb). The Donor Vector box, the Gateway® BP Clonase® II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items.

the intermediate entry clone, refer to the Gateway® Technology with Clonase® II manual for a one-tube protocol. Although this protocol allows you to generate expression clones more rapidly than the standard BP reaction followed by the LR reaction, fewer expression clones will be obtained (generally 10–20% of the total number of entry clones). Gateway Technology Clonase Ii Manual Gateway® Technology with Clonase® II A universal technology to clone DNA sequences for functional analysis and expression in. LR Clonase™ II enzyme mix is supplied as a 5X solution. If you wish to scale the reaction volume, make sure the LR Clonase™ II enzyme mix is at a final concentration of 1X. The Donor Vector box, the Gateway® BP Clonase® II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items. Store the Gateway® BP Clonase® II enzyme mix at −30°C to −10°C for up to 6 months.

Review and cite GATEWAY CLONING protocol, troubleshooting and other methodology THe manual (invitrogen) says to use the new LR clonase II plus. Gateway® Technology in manufacturing; (2) use of Gateway® Technology to provide a LR Clonase™ II Enzyme Mix to mediate the recombination reaction. 9 sept The LR reactions for two, three, and four fragment Gateway MultiSite recombination all use the LR Clonase II Plus enzyme mix.